Essay on FRET-Fluorescence resonance energy transfer
FRET Fluorescence resonance energy transfer (FRET) is a quantum process that occurs between two dye molecules. From the donor, molecule excitation is shifted to an acceptor fluorophore by the dipole-dipole interaction without photon emission. Consequently, quenching of donor molecule fluorescence results in the excitation of the acceptor molecule. Afterwards, it loses energy by the emission of heat or fluorescent known as sensitized emission. In FRET the intensity of donor fluorescence…